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Glucose Transport: Methods and Protocols (Methods in Molecular Biology)

Glucose Transport: Methods and Protocols (Methods in Molecular Biology)

Glucose Transport: Methods and Protocols (Methods in Molecular Biology) by Karin Lindkvist-Petersson
English | 8 Jan. 2018 | ISBN: 1493975064 | 288 Pages | PDF (True) | 7.28 MB
This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose

This volume explores methods used to examine the structure-function relationship of glucose transporters both in vitro and ex vivo. Chapters in this book cover topics such as expression and purification of glucose transporters in heterologous expression systems, crystal structure determination, glucose transport activity assays in cellular and synthetic model systems, investigation of the GLUT4 membrane trafficking machinery by novel labeling strategies combined with advanced fluorescence microscopy methods, and tracking GLUT2 translocation by live cell imaging. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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